LOCUS       40924_17354        3960 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEMBL18 plus DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Okamoto S, Niki H.
  TITLE     NBRP cloning vector collection sequence project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2017) Contact:Sho Okamoto National Institute of 
            Genetics, Microbial Genetics Laboratory; 1111 Yata, Mishima, 
            Shizuoka 411-8540, Japan URL 
            :https://www.nig.ac.jp/labs/MicroGen/index.html
REFERENCE   3  (bases 1 to 3960)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3960)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-APR-2017) Contact:Sho Okamoto National Institute of Genetics, 
            Microbial Genetics Laboratory; 1111 Yata, Mishima, Shizuoka 
            411-8540, Japan URL :https://www.nig.ac.jp/labs/MicroGen/index.html"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    104..125
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        140..170
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    178..194
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     202..218
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    228..284
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(288..304)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      792..1305
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2052..2156
                     /label=AmpR promoter
     CDS             2157..3014
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3188..3776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
       61 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      121 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      181 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa ttcgagctcg
      241 gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt
      301 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      361 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      421 ttgcgcagcc tgaatggcga atggaanttc cagacgattg agcgtcaaaa tgtaggtatt
      481 tccatgagcg tttttcctgt tgcaatggct ggcggtaata ttgttctgga tattaccagc
      541 aaggccgata gtttgagttc ttctactcag gcaagtgatg ttattactaa tcaaagaagt
      601 attgcgacaa cggttaattt gcgtgatgga cagactcttt tactcggtgg cctcactgat
      661 tataaaaaca cttctcagga ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc
      721 ctcctgttta gctcccgctc tgattctaac gaggaaagca cgttatacgt gctcgtcaaa
      781 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      841 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      901 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      961 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1021 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     1081 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     1141 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     1201 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttaaa tatttgctta
     1261 tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtacata tgattgacat
     1321 gctagtttta cgattaccgt tcatcgattc tcttgtttgc tccagactct caggcaatga
     1381 cctgatagcc tttgtagaga cctctcaaaa atagctaccc tctccggcat gaatttatca
     1441 gctagaacgg ttgaatatca tattgatggt gatttgactg tctccggcct ttctcacccg
     1501 tttgaatctt tacctacaca ttactcaggc attgcattta aaatatatga gggttctaaa
     1561 aatttttatc cttgcgttga aataaaggct tctcccgcaa aagtattaca gggtcataat
     1621 gtttttggta caaccgattt agctttatgc tctgaggctt tattgcttaa ttttgctaat
     1681 tctttgcctt gcctgtatga tttattggat gttggaantt cctgatgcgg tattttctcc
     1741 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     1801 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     1861 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     1921 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     1981 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     2041 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2101 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2161 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     2221 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     2281 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     2341 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     2401 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2461 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     2521 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     2581 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2641 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2701 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2761 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2821 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2881 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2941 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3001 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3061 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3181 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3241 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3301 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3361 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3421 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3481 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3541 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3601 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3661 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3721 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3781 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3841 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3901 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
//
